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Flavobacterium Medium (ATCC Medium 1687)

[所属分类:培养基配方] [发布时间:2021-11-18] [发布人:网站管理员2] [阅读次数:] [返回]

Flavobacterium Medium

(ATCC Medium 1687)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium glutamate ..............................................4.0g

K2HPO4............................................................0.65g

NaNO3................................................................0.5g

KH2PO4............................................................0.19g

MgSO4·7H2O .....................................................0.1g

FeSO4 solution ............................................... 2.0mL

pH 7.4 ± 0.2 at 25°C

FeSO4 Solution:

Composition per 10.0mL:

FeSO4·7H2O..................................................... 0.03g

Preparation of FeSO4 Solution: Add FeSO4 to

distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept FeSO4 solution, to distilled/deionized water and

bring volume to 998.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

25°C. Aseptically add 2.0mL of sterile FeSO4 solu

tion. Mix thoroughly. Adjust pH to 7.4. Aseptically

distribute into sterile tubes or flasks.

Use: For the cultivation of Flavobacterium species.