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Fish Peptone Broth

[所属分类:培养基配方] [发布时间:2021-11-18] [发布人:网站管理员2] [阅读次数:] [返回]

Fish Peptone Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Maltose .............................................................. 5.0g

NaCl................................................................... 5.0g

Peptone .............................................................. 5.0g

Pancreatic digest of casein.................................5.0g

Yeast extract.......................................................5.0g

Trout tissue extract solution......................... 50.0mL

pH 7.0 ± 0.2 at 25°C

Trout Tissue Extract Solution:

Composition per liter:

Fish (brook trout)...........................................500.0g

Pepsin.................................................................1.0g

HCl, concentrated ........................................ 15.0mL

Preparation of Trout Tissue Extract: Add 1.0L

of distilled/deionized water to brook trout and blend

for 20–30 min. Add 1.0g of pepsin and 15.0mL of con

centrated HCl to digest the trout proteins. Incubate for

12 hr at 45°C. Adjust pH to 7.0. Allow solids to settle.

Filter sterilize. Do not autoclave. Store at 5°C.

Preparation of Medium: Add components, except

trout tissue extract, to distilled/deionized water and

bring volume to 950.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 10 psi

pressure–118°C. Cool to 45°–50°C. Aseptically add

50.0mL of sterile trout tissue extract. Mix thoroughly.

Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Aeromonas salmonicida.