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Fermentation Broth (CHO Medium)

[所属分类:培养基配方] [发布时间:2021-11-18] [发布人:网站管理员2] [阅读次数:] [返回]

Fermentation Broth

(CHO Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................... 15.0g

Yeast extract....................................................... 7.0g

NaCl................................................................... 2.5g

Agar ................................................................. 0.75g

Sodium thioglycolate......................................... 0.5g

L-Cystine .......................................................... 0.25g

Ascorbic acid ..................................................... 0.1g

Bromthymol Blue ............................................ 0.01g

Carbohydrate or starch solution................. 100.0mL

pH 7.0 ± 0.1 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate...................................................... 6.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Starch Solution:

Composition per 100.0mL:

Starch ................................................................. 2.5g

Preparation of Starch Solution: Add starch to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 900.0mL. Mix thoroughly.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti

cally add 100.0mL of sterile carbohydrate solution.

Mix thoroughly. Aseptically distribute into sterile

tubes or flasks. Loosen caps on tubes. Place in an

anaerobic chamber under an atmosphere of 85% N2,

10% H2, and 5% CO2. Fasten the caps securely or

maintain in an anaerobic chamber.

Use: For the differentiation of anaerobic bacteria

based upon carbohydrate fermentation. Bacteria that

ferment carbohydrates turn the medium yellow.