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Fecal Coliform Agar, Modified

[所属分类:培养基配方] [发布时间:2021-11-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar .................................................................15.0g

Lactose.............................................................12.5g

Tryptose ...........................................................10.0g

Proteose peptone No. 3 ......................................5.0g

NaCl...................................................................5.0g

Yeast extract.......................................................3.0g

Bile salts No. 3...................................................1.5g

Aniline Blue.......................................................0.1g

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components and

bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Do not autoclave. Cool to 50°C.

Adjust pH to 7.4. Pour into sterile Petri dishes in

20.0mL volumes. Allow surface of plates to dry be

fore using.

Use: For the isolation, cultivation, and identification

of stressed fecal coliform microorganisms based on

their ability to ferment lactose. Lactose-fermenting

bacteria turn the medium blue.