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FC Broth (Fecal Coliform Broth) (m-FC Broth) (m-Fecal Coliform Broth)

[所属分类:培养基配方] [发布时间:2021-11-18] [发布人:网站管理员2] [阅读次数:] [返回]

FC Broth

(Fecal Coliform Broth)

(m-FC Broth)

(m-Fecal Coliform Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Lactose.............................................................12.5g

Tryptose ........................................................... 10.0g

NaCl................................................................... 5.0g

Proteose peptone No. 3...................................... 5.0g

Yeast extract....................................................... 3.0g

Bile salts............................................................. 1.5g

Aniline Blue....................................................... 0.1g

Rosolic acid solution.................................... 10.0mL

pH 7.4 ± 0.2 at 25°C

Rosolic Acid Solution:

Composition per 100.0mL:

Rosolic acid ....................................................... 1.0g

Preparation of Rosolic Acid Solution: Add ro

solic acid to 0.2N NaOH and bring volume to 100.0L.

Mix thoroughly.

Preparation of Medium: Add 10.0mL of rosolic

acid solution to 950.0mL of distilled/deionized wa

ter. Mix thoroughly. Add other components and bring

volume to 1.0L with distilled/deionized water. Mix

thoroughly. Gently heat and bring to boiling with fre

quent mixing. Do not autoclave. Pour into sterile Pet

ri dishes or leave in tubes.

Use: For the enumeration of coliform bacteria using

the membrane filtration method.