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Enrichment Broth, pH 7.3 with Pyruvate

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................17.0g

NaCl....................................................................5.0g

Papaic digest of soybean meal............................3.0g

Glucose ...............................................................2.5g

K2HPO4...............................................................2.5g

Yeast extract........................................................6.0g

Pyruvate solution .......................................... 11.1mL

Nalidixic acid solution.................................... 8.0mL

Cycloheximide solution .................................. 5.1mL

Acriflavin·HCl solution .................................. 2.0mL

pH 7.3 ± 0.2 at 25°C

Pyruvate Solution:

Composition per 20.0mL:

Sodium pyruvate.................................................2.0g

Preparation of Pyruvate Solution: Add sodium

pyruvate to distilled/deionized water and bring vol

ume to 20.0mL. Mix thoroughly. Filter sterilize.

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide....................................................0.1g

Ethanol, absolute............................................. 4.0mL

Preparation of Cycloheximide Solution: Add

components to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Nalidixic Acid Solution:

Composition per 10.0mL:

Nalidixic acid....................................................0.05g

Preparation of Nalidixic Acid Solution: Add

nalidixic acid to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Acriflavin·HCl Solution:

Composition per 10.0mL:

Acriflavin·HCl ..................................................0.05g

Preparation of Acriflavin·HCl Solution: Add

acriflavin·HCl to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex

cept pyruvate solution, cycloheximide solution, nali

dixic acid solution, and acriflavin·HCl solution—to

distilled/deionized water and bring volume to

973.8mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add 11.1mL of

sterile pyruvate solution. Mix thoroughly. Inoculate

medium and incubate at 30°C for 6 hr. Aseptically

add 5.1mL of sterile cycloheximide solution, 8.0mL

of sterile nalidixic acid solution, and 2.0mL of sterile

acriflavin·HCl solution. Mix thoroughly.

Use: For the isolation, cultivation, and enrichment of

a Listeria species from milk and dairy products.

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Enrichment Broth, pH 7.3 with Pyruvate