Andrade’s Carbohydrate Broth and Indicator (BAM M13)

[所属分类:培养基配方] [发布时间:2019-10-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Andrade’s Carbohydrate Broth and Indicator
(BAM M13)
Composition per liter:
Pancreatic digest of gelatin................................................10.0g
NaCl.........................................................................10.0g
Beef extract..................................................................3.0g
Carbohydrate solution.........................................................100.0mL
Andrade’s indicator...........................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BBL
Microbiology Systems, in tubes containing adonitol, arabinose, cello-
biose, glucose, dulcitol, fructose, galactose, inositol, lactose, maltose,
mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose, trehalose, or
xylose.
Andrade’s Indicator
Composition per 26.0mL :
NaOH (1N solution)........................................................16.0mL
Acid Fuchsin...............................................................0.21g
Preparation of Andrade’s Indicator: Add Acid Fuchsin to
NaOH solution and bring volume to 26.0mL with distilled/deionized
water.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate............................................................5.0–10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. For glucose,
lactose, sucrose, and mannitol, add 10.0g to distilled/deionized water
and bring volume to 100.0mL. For dulcitol, salicin, and other carbohy-
drates, add 5.0g to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except carbohydrate so-
lution, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Cool. Aseptically add 100mL of
sterile carbohydrate solution to 900mL of sterile medium. Mix thoroughly.
Aseptically distribute into tubes or flasks. Alternately, prior to autoclaving,
distribute 9.0mL volumes into test tubes containing inverted Durham
tubes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Add
1.0mL of sterile carbohydrate solution to each tube.
Caution: Acid Fuchsin is a potential carcinogen and care must be tak-
en to avoid inhalation of the powdered dye and contact with the skin.
Use: For the determination of carbohydrate fermentation reactions of
microorganisms, particularly members of the Enterobacteriaceae. A Dur-
ham tube is used to collect gas produced during the fermentation reaction.
Acid production is indicated by a pink color.