Andrade Peptone Water

[所属分类:培养基配方] [发布时间:2019-10-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Andrade Peptone Water
Composition per liter:
Peptic digest of animal tissue...............................................10.0g
NaCl..........................................................................5.0g
Andrade indicator ............................................................0.1g
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Caution: Acid Fuchsin in Andrade indicator is a potential carcinogen
and care must be taken to avoid inhalation of the powdered dye and
contact with the skin.
Use: For the determination of carbohydrate fermentation reactions of
microorganisms, particularly members of the Enterobacteriaceae. A spe-
cific carbohydrate is added to the medium to test the fermentation of that
carbohydrate. A Durham tube is used to collect gas produced during the
fermentation reaction. Acid production is indicated by a pink color.