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Bismuth Sulfite Agar Wilson and Blair (BAM M19)

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

Bismuth Sulfite Agar Wilson and Blair

(BAM M19)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Pancreatic digest of casein................................10.0g

Bi2(SO3)3.............................................................8.0g

Beef extract.........................................................5.0g

Glucose ...............................................................5.0g

Na2HPO4 .............................................................4.0g

FeSO4·7H2O........................................................0.3g

Brilliant Green ................................................0.025g

pH 7.7 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly and heat with frequent agitation until

boiling. Boil for 1 min. Do not autoclave. Cool to

45°–50°C. Pour into sterile Petri dishes while gently

shaking flask to disperse precipitate. Let plates dry

for about 2 hr with lids partially removed. Use plates

the within one day of preparation; medium loses se

lectivity after 48 hr.

Use: For the selective isolation and identification of

Salmonella typhi and other enteric bacilli. Salmonel

la typhi appears as flat, black, “rabbit-eye” colonies

surrounded by a zone of black with a metallic sheen.