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Bicarbonate Agar

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

Bicarbonate Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100.0mL:

Soybean-casein digest agar........................... 90.0mL

Sodium bicarbonate solution ........................ 10.0mL

pH 7.3 ± 0.2 at 25°C

Soybean-Casein Digest Agar:

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein................................15.0g

Papaic digest of soybean meal............................5.0g

NaCl....................................................................5.0g

Preparation of Soybean-Casein Digest Agar:

Add components to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C.

Sodium Bicarbonate Solution:

Composition per 10.0mL:

NaHCO3 ..............................................................0.7g

Preparation of Sodium Bicarbonate Solu

tion: Add NaHCO3 to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize. Use freshly prepared solution.

Preparation of Medium: To 90.0mL of cooled,

sterile soybean-casein digest agar, aseptically add

10.0mL of sterile sodium bicarbonate solution. Mix

thoroughly. Pour into sterile Petri dishes.

Use: For the cultivation of Vibrio species from

foods.