Ancalomicrobium adetum Medium

[所属分类:培养基配方] [发布时间:2019-10-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ancalomicrobium adetum Medium
Composition per liter:
Ammonium sulfate .....................................................................0.25g
Glucose.......................................................................................0.25g
Na2HPO4 ..................................................................................71.0mg
Modified Hutner’s basal salts..................................................20.0mL
Vitamin solution.......................................................................10.0mL
Modified Hutner’s Basal Salts:
Composition per liter:
MgSO4 ·7H2O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl2 ·2H2O................................................................................3.34g
FeSO4 ·7H2O............................................................................99.0mg
Ammonium molybdate............................................................9.25mg
Metals “44”..............................................................................50.0mL
Preparation of Modified Hutner’s Basal Salts:  Dissolve the
nitrilotracetic acid first and neutralize the solution with KOH. Add oth-
er components and adjust the pH to 7.2 with KOH or H 2 SO 4 . There
may be a slight precipitate. Store at 5°C.
Metals “44”
Composition per 100.0mL:
ZnSO4 ·7H2O.................................................................................1.1g
FeSO4 ·7H2O .................................................................................0.5g
CuSO4 ·5H2O...............................................................................0.04g
EDTA..........................................................................................0.25g
MnSO4 ·7H2O............................................................................0.154g
Co(NO3)2 ·6H2O........................................................................0.025g
Na2B4O7 ·10H2O.......................................................................0.018g
Preparation of Metals “44”:  Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Add aseptically to sterile modi-
fied Hutner’s basal salts solution.
Vitamin Solution:
Composition per liter:
Thiamine HCI...............................................................................5.0g
Calcium  DL -pantothenate...........................................................5.0mg
Nicotinamide..............................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except modified Hut-
ner’s basal salts solution and vitamin solution, to distilled/deionized
water and bring volume to 970.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Aseptically add 20.0mL of modified
Hutner’s basal salts solution and 10.0mL of sterile vitamin solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Ancalomicrobium adetum.