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AKI Medium (BAM M7)

[所属分类:培养基配方] [发布时间:2021-10-28] [发布人:网站管理员2] [阅读次数:] [返回]

AKI Medium

(BAM M7)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .............................................................15.0g

NaCl....................................................................5.0g

Yeast extract........................................................4.0g

Sodium bicarbonate solution ........................ 30.0mL

pH 7.2 ± 0.2 at 25°C

Sodium Bicarbonate Solution:

Composition per 100.0mL:

NaHCO3............................................................10.0g

Preparation of Sodium Bicarbonate Solu

tion: Add sodium bicarbonate to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly.

Filter sterilize. Use freshly prepared solution.

Preparation of Medium: Add components, except

sodium bicarbonate solution, to distilled/deionized wa

ter and bring volume to 970.0mL. Mix thoroughly. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile sodium bicarbonate

solution. Mix thoroughly. Aseptically distribute into

sterile tubes or flasks. Prepare medium freshly.

Use: For the cultivation of Vibrio cholerae and other

Vibrio species.