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Agar Medium P (PM Indicator Agar) (BAM M6)

[所属分类:培养基配方] [发布时间:2021-10-28] [发布人:网站管理员2] [阅读次数:] [返回]

Agar Medium P

(PM Indicator Agar)

(BAM M6)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Glucose .............................................................5.25g

Peptone................................................................5.0g

Beef extract.........................................................3.0g

Pancreatic digest of casein..................................1.7g

Tween 80.............................................................1.0g

NaCl....................................................................0.5g

Papaic digest of soybean meal............................0.3g

K2HPO4 ............................................................0.25g

Bromcresol Purple ............................................0.06g

pH 7.8 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the cultivation of Bacillus stearothermo

philus for the detection of penicillin in milk.