Anaerobranca Medium

[所属分类:培养基配方] [发布时间:2019-10-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobranca Medium

Composition per 1015.0mL:
Yeast extract..................................................................................5.0g
Na2HPO4 ·2H2 O.............................................................................3.9g
Sodium fumarate...........................................................................1.5g
KCl................................................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
L -Cysteine·HCl·H 2 O.................................................................0.125g
Na 2 S·9H 2 O................................................................................0.125g
Wolfe’s vitamin solution..........................................................10.0mL
Wolfe’s mineral solution............................................................5.0mL
pH 8.5 ± 0.2 at 25°C
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
p-Aminobenzoic acid.................................................................5.0mg
Lipoic acid.................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
Riboflavin..................................................................................5.0mg
Thiamine·HCl............................................................................5.0mg
Calcium  DL -pantothenate...........................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Wolfe’s Vitamin Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Wolfe’s Mineral Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H2O................................................................................0.5g
CoCl2 ·6H2O..................................................................................0.1g
ZnSO4 ·7H2O.................................................................................0.1g
CaCl2 ·2H2O ..................................................................................0.1g
FeSO4 ·7H2O..................................................................................0.1g
NiCl2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H2 O.......................................................................0.02g
CuSO4 ·5H2O...............................................................................0.01g
H3BO 3 .........................................................................................0.01g
Na MoO 4 ·2H 2 O..........................................................................0.01g
Na2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components. Add distilled/deionized water to
1.0L. Adjust pH to 6.8.
Preparation of Medium: Prepare and dispense medium under
100% N 2 . Add components, except Wolfe’s vitamin solution, to dis-
tilled/deionized water and bring volume to 990.0mL. Mix thoroughly.
Adjust pH to 8.5. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically and anaerobically add 10.0mL of sterile
Wolfe’s vitamin solution. Mix thoroughly. Aseptically and anaerobi-
cally distribute into sterile tubes or bottles.
Use: For the cultivation of Anaerobranca horikoshii.