Anaerobic TVLS Medium

[所属分类:培养基配方] [发布时间:2019-10-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobic TVLS Medium
Composition per liter:
Pancreatic digest of casein..........................................................17.0g
Beef extract...................................................................................7.5g
Glucose .........................................................................................6.0g
Enzymatic hydrolysate of soybean meal ......................................3.0g
Liver hydrolysate..........................................................................3.0g
NaCl..............................................................................................2.5g
Na2SO3 ..........................................................................................0.7g
Sodium thioglycolate....................................................................0.5g
L- Cysteine·HCl·H2O....................................................................0.25g
Agar ..............................................................................................0.1g
Bovine serum.........................................................................100.0mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except bovine serum,
to distilled/deionized water and bring volume to 900.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of bo-
vine serum. Distribute into sterile tubes.
Use: For the isolation and cultivation of anaerobic microorganisms.