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Y 1 Adrenal Cell Growth Medium

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 580.0mL:

Ham’s F-10 medium ..............................................................500.0mL

Fetal bovine serum...................................................................75.0mL

Penicillin-streptomycin solution ................................................5.0mL

pH 7.0 ± 0.2 at 25°C

Ham’s F-10 Medium:

Composition per liter:

NaCl .............................................................................................. 7.4g

NaHCO3 ........................................................................................ 1.2g

Glucose ......................................................................................... 1.1g

NaH2PO4·H2O............................................................................. 0.29g

KCl.............................................................................................. 0.28g

L-Arginine·HCl............................................................................ 0.21g

L-Glutamine................................................................................. 0.15g

MgSO4·7H2O .............................................................................. 0.15g

Sodium pyruvate ......................................................................... 0.11g

KH2PO4....................................................................................... 0.08g

CaCl2·2H2O................................................................................. 0.04g

L-Cystine·2HCl............................................................................ 0.04g

L-Histidine·HCl·H2O................................................................... 0.02g

L-Lysine·HCl ............................................................................... 0.02g

L-Asparagine-H2O....................................................................... 0.01g

L-Aspartic Acid ........................................................................... 0.01g

L-Glutamic acid ........................................................................... 0.01g

L-Leucine..................................................................................... 0.01g

L-Proline...................................................................................... 0.01g

L-Serine ....................................................................................... 0.01g

L-Alanine....................................................................................8.9mg

Glycine.......................................................................................7.5mg

D-Phenylalanine..........................................................................5.0mg

L-Methionine ..............................................................................4.5mg

Hypoxanthine.............................................................................4.1mg

L-Threonine ................................................................................3.6mg

L-Valine ..................................................................................... 3.5mg

L-Isoleucine ................................................................................2.6mg

L-Tyrosine...................................................................................1.8mg

Vitamin B12 ................................................................................1.4mg

Folic acid....................................................................................1.3mg

Phenol Red.................................................................................1.2mg

Thiamine·HCl ............................................................................1.0mg

FeSO4·7H2O...............................................................................0.8mg

Choline chloride.........................................................................0.7mg

D-Calcium pantothenate .............................................................0.7mg

Thymidine..................................................................................0.7mg

Niacinamide ...............................................................................0.6mg

L-Tryptophan ..............................................................................0.6mg

Isoinositol...................................................................................0.5mg

Riboflavin ..................................................................................0.4mg

Lipoic acid .................................................................................0.2mg

Pyridoxine·HCl ..........................................................................0.2mg

ZnSO4·7H2O ............................................................................0.03mg

Biotin .......................................................................................0.02mg

CuSO4·5H2O ...............................................................................3.0μg

Preparation of Ham’s F-10 Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Penicillin-Streptomycin Solution:

Composition per 100.0mL:

Streptomycin................................................................................. 0.5g

Penicillin G .......................................................................... 500,000U

Preparation of Penicillin-Streptomycin Solution: Add compo

nents to distilled/deionized water and bring volume to 100.0mL. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine components. Filter

sterilize. Store at 4°–5°C.

Use: For the cultivation of Y-1 mouse adrenal tissue culture cells used for

the detection of cholera enterotoxin (CT) produced by enterotoxigenic

strains of Vibrio cholerae or Vibrio mimicus. CT causes the conversion of

elongated fibroblast-like cells into round, refractile cells.

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Y 1 Adrenal Cell Growth Medium