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XPS Broth with Thymidine (Thymidine Auxotroph XPS Medium)

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

XPS Broth with Thymidine

(Thymidine Auxotroph XPS Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Potatoes, infusion from............................................................... 40.0g

Sucrose........................................................................................ 15.0g

Peptone ......................................................................................... 5.0g

Glucose ......................................................................................... 4.0g

Casamino acids ............................................................................. 1.0g

Na2HPO4..................................................................................... 0.79g

Thymidine................................................................................ 10.0mg

Ca(NO3)2·4H2O solution .........................................................10.0mL

pH 5.1 ± 0.2 at 25°C

Potatoes, Infusion From:

Composition per 500.0mL:

Potatoes......................................................................................... 4.0g

Preparation of Potatoes, Infusion From: Peel and dice potatoes.

Add 500.0mL of distilled/deionized water. Gently heat and bring to

boiling. Continue boiling for 30 min. Filter through cheesecloth.

Ca(NO3)2·4H2O Solution:

Composition per 10.0mL:

Ca(NO3)2·4H2O ............................................................................ 0.5g

Preparation of Ca(NO3)2·4H2O Solution: Add Ca(NO3)2·4H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components, except Ca(NO3)2·4H2O

solution, to distilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Asep

tically add 10.0mL of sterile Ca(NO3)2·4H2O solution. Mix thorough

ly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Xanthomonas oryzae.