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XLD Agar, HiVeg (Xylose Lysine Deoxycholate HiVeg Agar)

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

XLD Agar, HiVeg

(Xylose Lysine Deoxycholate HiVeg Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Lactose .......................................................................................... 7.5g

Sucrose.......................................................................................... 7.5g

Na2S2O3 ........................................................................................ 6.8g

L-Lysine ........................................................................................ 5.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 4.0g

Xylose........................................................................................... 3.5g

Synthetic detergent No. III ........................................................... 1.5g

Ferric ammonium citrate............................................................... 0.8g

Phenol Red.................................................................................. 0.08g

Selective supplement solution ...................................................4.6mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Selective Supplement Solution:

Composition per 100.0mL:

Tergitol™ 4........................................................................ Proprietary

Preparation of Selective Supplement Solution: Available as pre

mixed solution.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Do not overheat. Distribute into tubes or flasks. Do not au

toclave. Pour into sterile Petri dishes or leave in tubes. Plates should be

poured as soon as possible to avoid precipitation.

Use: For the isolation and differentiation of enteric pathogens, espe

cially Shigella and Providencia species. Nonfermenting xylose/lac

tose/sucrose bacteria appear as red colonies. Xylose-fermenting,

lysine-decarboxylating bacteria appear as red colonies. Xylose-fer

menting, lysine-nondecarboxylating bacteria appear as opaque yellow

colonies. Lactose- or sucrose-fermenting bacteria appear as yellow col

onies.