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Xanthobacter agilis Agar

[所属分类:培养基配方] [发布时间:2021-10-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Solution A.....................................................................................1.0L

Solution B ..............................................................................100.0mL

Solution A:

Composition per liter:

Agar ............................................................................................ 15.0g

NaH2PO4·12H2O........................................................................... 9.0g

KH2PO4......................................................................................... 1.5g

NH4·Cl .......................................................................................... 1.0g

Sodium propionate or 3-hydroxybutyrate..................................... 1.0g

MgSO4·7H2O ................................................................................ 0.2g

Trace elements solution .............................................................1.0mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution:

Composition per 2.0mL:

H3BO4 .....................................................................................560.0μg

ZnSO4·7H2O ...........................................................................350.0μg

NiCl2·H2O ...............................................................................160.0μg

Na2MoO4·2H2O ......................................................................100.0μg

CuSO4·5H2O .............................................................................16.0μg

MnCl2·4H2O..............................................................................16.0μg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 2.0mL. Mix thoroughly.

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C.

Solution B:

Composition per 100.0mL:

Ferric ammonium citrate..........................................................50.0mg

CaCl2·2H2O............................................................................100.0mg

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically combine 1.0L of sterile solu

tion A with 100.0mL of sterile solution B. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Xanthobacter agilis.