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Wort Agar 6°Brix

[所属分类:培养基配方] [发布时间:2021-10-14] [发布人:网站管理员2] [阅读次数:] [返回]

Wort Agar 6°Brix

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Malt........................................................................................... 250.0g

Agar ............................................................................................ 20.0g

Preparation of Medium: Add 250.0g of ground malt to tap water

and bring volume to 1.0L. Mix thoroughly. Gently heat to 55°–60°C.

Maintain at 55°–60°C for 1.5–2.0 hr while mixing regularly. Elevate

temperature to 80°C and maintain for 10 min with thorough mixing.

Cool the wort to 25°C and filter through a linen bag. Adjust the con

centration of sugars to 6° Brix (specific gravity of 1.024 at 24°C). Add

20.0g of agar. Gently heat and bring to boiling. Mix thoroughly. Dis

tribute into tubes or flasks. Autoclave for 30 min at 3 psi pressure–

105°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Rhodococcus erythropo

lis.