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Wort Agar

[所属分类:培养基配方] [发布时间:2021-10-14] [发布人:网站管理员2] [阅读次数:] [返回]

Wort Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Malt extract ................................................................................. 15.0g

Maltose...................................................................................... 12.75g

Dextrin ........................................................................................ 2.75g

Glycerol ...................................................................................... 2.35g

K2HPO4......................................................................................... 1.0g

NH4Cl ........................................................................................... 1.0g

Pancreatic digest of gelatin ......................................................... 0.78g

pH 4.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Boil for 1 min with mixing. Distribute into tubes or flasks. Au

toclave for 15 min at 15 psi pressure–121°C. Do not overheat, as this will

result in hydrolysis of the agar. An additional 5.0g of agar can be used to

make a firmer agar. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and enumeration of yeasts. The low pH of the

agar selectively inhibits bacterial growth.