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WL Nutrient Agar (Wallerstein Laboratory Nutrient Agar)

[所属分类:培养基配方] [发布时间:2021-10-14] [发布人:网站管理员2] [阅读次数:] [返回]

WL Nutrient Agar

(Wallerstein Laboratory Nutrient Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 50.0g

Agar ............................................................................................ 20.0g

Pancreatic digest of casein............................................................ 5.0g

Yeast extract.................................................................................. 4.0g

KH2PO4....................................................................................... 0.55g

KCl............................................................................................ 0.425g

CaCl2·2H2O .............................................................................. 0.125g

MgSO4·7H2O............................................................................ 0.125g

Bromcresol Green..................................................................... 0.022g

FeCl3 .......................................................................................... 2.5mg

MnSO4·4H2O............................................................................. 2.5mg

Na2CO3 (1% solution)............................................................30.0mL

pH 6.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat with mix

ing and bring to boiling. Distribute into tubes or flasks. Autoclave for

15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave

in tubes.

Use: For the detection of bacteria and yeasts from industrial fermenta

tion processes, particularly from beer processing.