培养基配方

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Winogradsky’s N-Free Medium

[所属分类:培养基配方] [发布时间:2021-10-13] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

CaCO3 ........................................................................................ 5.0mg

Sugar solution ........................................................................100.0mL

Concentrated salt solution..........................................................5.0mL

pH 7.2 ± 0.2 at 25°C

Sugar Solution:

Composition per 100.0mL:

Sucrose or glucose ...................................................................... 10.0g

Preparation of Sugar Solution: Add sucrose or glucose to 100.0mL

of distilled/deionized water. Mix thoroughly. Autoclave for 10 min at 10 psi

pressure–115°C. Cool to 50°C.

Concentrated Salt Solution:

Composition per liter:

KH2PO4....................................................................................... 50.0g

MgSO4·7H2O.............................................................................. 25.0g

NaCl............................................................................................ 25.0g

FeSO4·7H2O.................................................................................. 1.0g

MnSO4·4H2O................................................................................ 1.0g

Na2MoO4·4H2O ............................................................................ 1.0g

Preparation of Concentrated Salt Solution: Add components

to tap water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except sugar solution,

to distilled/deionized water and bring volume to 900.0mL. Mix thor

oughly. Gently heat and bring to boiling. Distribute into tubes or flasks.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti

cally add sugar solution. Adjust pH to 7.2. Mix thoroughly. Pour into

sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Azomonas insignis.