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Wilkins-Chalgren Anaerobe Agar with Cysteine

[所属分类:培养基配方] [发布时间:2021-10-13] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 10.0g

Pancreatic digest of casein.......................................................... 10.0g

Gelatin peptone........................................................................... 10.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 5.0g

Glucose ......................................................................................... 1.0g

L-Arginine ..................................................................................... 1.0g

Sodium pyruvate........................................................................... 1.0g

L-Cysteine·HCl ............................................................................. 0.3g

Hemin ........................................................................................ 5.0mg

Menadione ................................................................................. 0.5mg

Defibrinated blood...................................................................50.0mL

Tween™ 80................................................................................1.0mL

pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components, except defibrinated

blood, to distilled/deionized water and bring volume to 950.0mL. Mix

thoroughly. Gently heat and bring to boiling. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–

55°C. Aseptically add 50.0mL of defibrinated blood. Mix thoroughly.

Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Agromonas oligotrophica, Falcivibrio gran

dis, and Falcivibrio vaginalis.