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Wheat Peptone Agar

[所属分类:培养基配方] [发布时间:2021-10-13] [发布人:网站管理员2] [阅读次数:] [返回]

Wheat Peptone Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 750.0mL:

Agar ............................................................................................ 20.0g

Peptone........................................................................................ 20.0g

Yeast extract................................................................................ 10.0g

Glycerol ...................................................................................... 10.0g

Wheat grain extract................................................................500.0mL

pH 6.8–7.0 at 25°C

Wheat Grain Extract:

Composition per 500.0mL:

Whole wheat ............................................................................... 30.0g

Preparation of Wheat Grain Extract: Add whole wheat to tap wa

ter and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring

to boiling. Continue boiling for 60 min. Filter through several thicknesses

of cheesecloth padded with cotton.

Preparation of Medium: Add agar, peptone, yeast extract, and

glycerol to whole grain extract filtrate. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or aseptically distribute into sterile tubes.

Use: For the cultivation and utilization of Septoria nodorum.