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Walsby Medium

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

Walsby Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

MgSO4·7H2O............................................................................ 0.075g

K2HPO4..................................................................................... 0.039g

Na2CO3 ....................................................................................... 0.02g

CaCl2·2H2O .............................................................................. 0.018g

H3BO3 ........................................................................................ 2.8mg

MnSO4·4H2O............................................................................. 2.0mg

ZnSO4 ...................................................................................... 0.22mg

MoO3 ....................................................................................... 0.18mg

CuSO4·5H2O............................................................................ 0.08mg

Co(NO3)2·6H2O ....................................................................... 0.05mg

Iron-EDTA solution ...................................................................1.0mL

pH 8.5 ± 0.2 at 25°C

Iron-EDTA Solution:

Composition per liter:

EDTA.......................................................................................... 12.7g

FeSO4·7H2O................................................................................ 4.98g

Preparation of Iron-EDTA Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of planktonic gas-vacuolate

cyanobacteria.