培养基配方

您现在的位置：网站首页 >> 培养基配方

Wall Defective Bacterial Medium

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose...................................................................................... 100.0g

Papaic digest of soybean meal .................................................... 20.0g

Agarose ....................................................................................... 10.0g

Yeast extract................................................................................ 10.0g

NaCl .............................................................................................. 5.0g

MgSO4·7H2O ................................................................................ 2.5g

Horse serum ...........................................................................200.0mL

Cholesterol solution .................................................................10.0mL

pH 7.8 ± 0.2 at 25°C

Cholesterol Solution:

Composition per 10.0mL:

Cholesterol .................................................................................. 0.04g

Ethanol (95% solution) ............................................................10.0mL

Preparation of Cholesterol Solution: Add cholesterol to 10.0mL

of 95% ethanol. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except cholesterol so

lution and horse serum, to distilled/deionized water and bring volume

to 790.0mL. Mix thoroughly. Adjust pH to 7.8. Add 10.0mL of choles

terol solution. Mix thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Asepti

cally add 200.0mL of sterile horse serum. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of cell wall-deficient bacteria, such as L

forms, that depend on osmotic stabilization.