培养基配方

您现在的位置：网站首页 >> 培养基配方

VP HiVeg Medium

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

VP HiVeg Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

NaCl............................................................................................ 20.0g

Sucrose........................................................................................ 20.0g

Plant peptone .............................................................................. 10.0g

Sodium citrate............................................................................. 10.0g

Na2S2O3 ...................................................................................... 10.0g

Synthetic detergent No. V............................................................. 5.0g

Yeast extract.................................................................................. 5.0g

Sodium lauryl sulfate .................................................................... 0.2g

Bromthymol Blue ....................................................................... 0.04g

Thymol Blue ............................................................................... 0.04g

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.9.

Distribute into tubes in 3.0mL volumes. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the cultivation and differentiation of bacteria based on their

ability to produce acetoin.